2.1. Microalgae and culture conditions
Fatty SL-327 composition of Chlorella sp. KR-1.(Unit: FAME/cell (mg/g))Chlrollela sp. KR-1MyristateC14:00.380.40PentadecanoateC15:00.000.00PalmitateC16:076.3379.86PalmitoleateC16:10.840.90StearateC18:025.2226.45OleateC18:1n9c66.1068.38LinoleateC18:2n6c69.6371.91Gamma-linoleateC18:3n60.910.00LinoleateC18:3n3c16.9917.41Others38.4840.82Total294.87306.13Fame (%)30.05Full-size tableTable optionsView in workspaceDownload as CSV
2.2. Harvesting and lipid extraction
2.3. Experimental design
Response surface methodology (RSM) was taken to obtain an optimal condition of the prophase cell disruption method and to investigate the effect of critical variables including H2O2 concentration (A), reaction time (B), and temperature (C) on FeCl3-based extraction yield (Y1) and Fe2(SO4)3-based extraction yield (Y2). The adopted reaction conditions were as follows: H2O2 concentrations of 1–3%, reaction times of 30–90 min and temperatures of 80–120 °C, at the microalgae concentration of 20 g/L. A total of 17 experimental runs of the three variables were designed by Box–Behnken design using the Design-Expert software (Version 8.0, Stat-Ease, Inc., USA).