## Lipid content of Chlrollela sp KR

3.3. Fitting response data to model statistics

In this Retinyl glucoside study, a three-factor, three-level Box–Behnken statistical experimental design was used to optimize the formulation variables and the RSM requires 17 experiments. The experimental data, including independent variables and responses for all 17 experimental runs, are summarized in Table 3. On the basis of the analysis of the data of 17 runs of the experiments, the polynomial equations for responses Y1 (Eq. (1)) and Y2 (Eq. (2)) are as follows:equation(1)Y1=58.37+7.5A+5.14B+33.50C-0.59AB-3.21AC+1.63BC-1.29A2-1.58B2-2.44C2Y1=58.37+7.5A+5.14B+33.50C-0.59AB-3.21AC+1.63BC-1.29A2-1.58B2-2.44C2equation(2)Y2=65.28+3.79A+2.56B+15.35C-0.59AB-0.58AC-0.19BC+1.22A2+0.79B2+10.35C2Y2=65.28+3.79A+2.56B+15.35C-0.59AB-0.58AC-0.19BC+1.22A2+0.79B2+10.35C2

Table 3.

ANOVA for response surface quadratic mode analysis of variancea.ResponseModelLack of fitF-valuep-Value prob > FR2Adj. R2Pred. R2Adeq. Prec.C.V. (%)F-valuep-Value prob > FFeCl3-based extraction yield (%) Y1668.49<0.00010.99880.99730.985986.3052.283.700.1194Fe2(SO4)3-based extraction yield (%) Y2102.22<0.00010.99240.98270.931730.1122.331.430.3576aAdj. R2, adjusted R2; Pred. R2, predicted R2; Adeq. Prec., adequate precision; C.V., coefficient of variation; ‘Prob > F’ is rheumatoid arthritis the significance level and a value less than 0.05 considered significant.Full-size tableTable optionsView in workspaceDownload as CSV