2.3. Microbial CA074 analysis
Samples taken from each reactor on the date of the highest VFAs production were centrifuged at 13,000 rpm for 5 min (Labogene 1524, Denmark) and the supernatant was decanted. The remaining cell-pellet was resuspended with 0.5 mL of deionized and distilled water (DDW) and centrifuged again under the same condition mentioned above. The supernatant was discarded and the cell-pellet was resuspended with 100 μL of DDW. Total DNA was extracted and purified from the suspension utilizing a Nucleo Spin soil kit (Macherey–Nagel, Germany). The purified DNA was stored at −20 °C for subsequent analysis.
3. Results and discussion
3.1. Effect of temperature on VFAs production and composition
Fig. 1. Effect of operating temperature on total VFAs (TVFAs) concentration and the tropic hormone ratio of TVFAs to TCOD.Figure optionsDownload full-size imageDownload as PowerPoint slide
Fig. 2. Effect of operating temperature on (a) soluble protein and (b) soluble carbohydrate concentrations during the microalgae fermentation.Figure optionsDownload full-size imageDownload as PowerPoint slide