2.1. Characteristics of feedstocks and inoculum sludge
The seed sludge used in this study was harvested from thickener tank of wastewater treatment plant located in Alexandria city, Egypt. The mixed culture bacteria was further concentrated by settling for 24 h, and stored for one month under SCH527123 conditions. The characteristics of OFMSW, GSW, PMSW and the seed sludge are presented in Table 1.
2.2. Experimental set-up
Batch dry anaerobic co-digestion experiments for hydrogen production from OFMSW/GSW; OFMSW/PMS and OFMSW/GSW/PMS.Batches/substratesSeed sludge (mL)OFMSW (mL)GSW (mL)PMS (mL)OFMSW + GSW50100005075250505050050257505001000OFMSW + PMS50900105075025505005050250755000100OFMSW + GSW + PMS5090555080101050805155080155507020105070151550701020Full-size tableTable optionsView in workspaceDownload as CSV
2.3. Kinetic studies
Cumulative hydrogen production curves were recorded over the time course of the batch experiments and analyzed using the modified Gompertz equation (Elsamadony et al., 2015b):equation(1)H=Pexp-expRmeP(λ-t)+1where H (mL) is fats the cumulative hydrogen production at the reaction time t (h), P (mL) is the hydrogen potential, Rm (mL/h) is the maximum hydrogen production rate, e = 2.71828, and λ (h) is the lag phase period.