All chemicals used in this study were of high purity and were used without further treatment and purification. The canola oil feedstock used in this experimental work was produced by a commercial manufacturer (J-Oil Mills, Tokyo, Japan). Methanol (99.0%), ethanol (99.5%), and MTBE (99.5%) were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). The following standard compounds were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan): fatty Carboplatin methyl esters (FAME): methyl oleate (min. 60.0%), methyl linoleate (min. 98.0%), methyl linolenate (min. 95.0%), methyl palmitate (min. 97.0%), and methyl stearate (min. 95.0%); and fatty acid ethyl esters (FAEE): ethyl oleate (99.9%), ethyl linoleate (min. 97.0%), ethyl linolenate (min. 95.0%), ethyl palmitate (min. 95.0%), and ethyl stearate (min. 90.0%). Triolein (99.9%), diolein (99.9%), and monoolein (min. 40.0%) standards were purchased from Nacalai Tesque, Inc., Sigma–Aldrich, Co. (Japan), and Tokyo Chemical Industry Co., Ltd., respectively. To prepare GC standard solutions, analytical grade tricaprin and n-hexane were used.