With the adaptive evolution process of adsorption performance
Specific growth rate, μ = ((log10Z − log10Z0) 2.303)/(t − t0)
Z and Z0 corresponded to Alvespimycin cell growth at time t and t0, respectively.
Linearized Haldane equation, 1/μ = 1/μmax + (I/Kiμmax)
Linear graph was plotted with 1/μ versus initial inhibitor concentrations (S). Maximum specific growth rate (μmax) was obtained from the slope of the curve, and inhibition constant (Ki) was derived from intercept on X axis.
2.7. Hydrocarbon degradation by Pseudozyma
Hydrocarbon degradation by Pseudozyma sp. NII 8165 was studied by growing the pulmonary circuit yeast in BH medium containing diesel, kerosene and petrol as the carbon source at 30 °C, 200 rpm for 35 days. BH medium containing no carbon source, inoculated with the Pseudozyma sp. was taken as negative control. Sampling was performed at seven days interval to measure the cell dry weight. At the end of incubation, INT assay was performed as indicated above. Hydrocarbon utilization was studied by analysing the hydrocarbon using GC–MS.