Saponification experiments were performed with a constant amount of wet

Fatty RG2833 yields obtained by direct saponification of N. gaditana wet biomass: optimization of the KOH/biomass ratio (A) and the aqueous ethanol/biomass ratio (B).A) KOH/biomass ratio (g/g dry biomass)Recovery yield (wt %)a1.690.9 ± 1.61.287.8 ± 0.30.883.7 ± 0.80.489.4 ± 1.10.287.3 ± 0.4B) Aqueous ethanol/biomass ratio (mL/g dry biomass)Recovery yield (wt %)a1080.0 ± 1.81485.3 ± 4.52287.5 ± 0.53090.6 ± 0.73890.8 ± 0.85890.9 ± 0.17687.3 ± 0.730b87.6 ± 1.5Operational conditions: A) 24.5 g wet biomass (5 g dry biomass), 380 mL aqueous ethanol (76 mL/g dry biomass), 1 h, 60 °C. B) 24.5 g wet biomass, 1 g KOH (85%) (0.2 g/g dry biomass), 1 h, 60 °C.aPercentage of fatty acids extracted with respect to the fatty acids present in the initial biomass.bExperiment with 500 g of wet biomass.Full-size tableTable optionsView in workspaceDownload as CSV