Fig. 6. A hologram recorded by CCD.Figure optionsDownload full-size imageDownload as PowerPoint slide
Fig. 7. A reconstructed hologram.Figure optionsDownload full-size imageDownload as PowerPoint slide
As shown in Fig. 7, the particles in Fig. 6 can be substantially reconstructed, with Lapatinib high degree of recognition. Setting the gray threshold to be 0.5, the three-dimensional position and size distribution of particles can be calculated after binarization, as shown in Fig. 8. It can be seen in Fig. 8, the spacing of particles is cilia much greater than particle diameter. The minimum Z axis position of reconstructed particles is 8 μm, maximum is 500 μm. The span of Z axis position is 492 μm. The span of X axis position is 607 μm, and span of Y axis position is 457 μm. The volume of particle field is 1.365 × 10−10 m3.
Fig. 8. Distribution of particle spatial positions.Figure optionsDownload full-size imageDownload as PowerPoint slide