dapt secretase As previously mentioned white rot fungi have

Assessment of enzyme activities was carried out as previously reported (Ntougias et al., 2012). In brief, laccase activity (Lac) was measured colorimetrically at 425 nm by mixing 0.8 ml fungal extract with 0.4 ml 1.5 mM 2,2-amino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1.2 ml 0.1 M tartrate buffer at pH 4.5. The manganese-independent peroxidase (MnIP) activity was assessed at 590 nm by oxidizing 0.1 ml 1 mM 3-methyl-2-benzothiazoline hydrazone (MBTH) with 0.2 ml 25 mM 3-dimethylaminobenzoic γ-Secretase inhibitor IX (DMAB) in the presence of 0.66 ml fungal extract, 0.01 ml 10 mM H2O2 and 1 ml 0.1 M succinate-lactate buffer at pH 4.5. Calculations involved subtraction of background activity (estimated as above in the absence of H2O2). The manganese peroxidase (MnP) activity was estimated as reported for MnIP, but in the presence of 0.01 ml 20 mM MnSO4 and by subtracting MnIP activity from the initial value obtained. In addition, lignin peroxidase and veratryl alcohol oxidase activity assays were also carried out, although no such activities were detected in the fungi examined in the present study. For all enzyme activities measured, one unit was defined as the quantity of enzyme oxidizing 1 μmol substrate per min.