Peroxidase activities were only detected in the P. ostreatus and I. andersonii extracts, while the low peroxidases content in the T. versicolor CCBAS614 culture (“5 wks”) resulted in non-detectable pertinent activities in the respective “Extract” treatment. Furthermore, no peroxidases activities were observed when OMW was used as substrate for the growth of the selected wood-rot fungi (“Control-t9”), as well as in the “Blank”, “Blank-Laccase” and “Blank-H2O2” treatments ( Fig. 6). This Gefitinib in accordance with the outcome of previous studies reporting late induction of Mn-dependent and -independent peroxidases ( D’Annibale et al., 1998, Koutrotsios and Zervakis, 2014 and Ntougias et al., 2012).
The majority of the wood-rot fungi demonstrated high OMW’s degradation which was correlated to the production of laccases and peroxidases. Culture extracts from selected strains reduced OMW phenolics within a nine days period. Addition of catalase resulted in high OMW’s color reduction without affecting fungal enzyme activities. The effluent showed an inhibitory effect during the early stages of laccase production/addition, not observed in the case of peroxidases. Moreover, decrease in phenolics was associated with enzymatic oxidation since thermal treatment of OMW suppressed dephenolization. White-rot fungal culture extracts can be applied in OMW degradation due to their enzymes stability and activity.