Nowadays the energy crisis and environmental problems caused by the

5. Implications
Recent application developed in this TAI-1 study provides a novel approach to inhibit methanogenesis in MECs. More research should be considered in future.
AcknowledgementThis study was supported by the Scientific and Technological Research Council of Turkey (TÜB?TAK) (Project No. 113Z589).
Duckweed; Lemna aequinoctialis; Light intensity; Photoperiod; Starch production
1. Introduction
2. Methods
2.1. Duckweed strain and culture method
Lemna aequinoctialis 6000, which had the best ability to accumulate starch, was isolated from Lixian county in Hunan province by large-scale screening of more than 100 clones of duckweed distributed in 20 provinces in China ( Yu et al., 2014 and Yu et al., 2015). L. aequinoctialis 6000 was pre-cultured in a large tub (60 cm × 40 cm × 10 cm) for 2 weeks on 1/2 Schenk-Hildebrandt medium (SH) ( Schenk and Hildebrandt, 1972). The pH of the medium was adjusted to 5.80. The tub was placed into a 23 °C growth chamber with the photoperiod of 16:8 and light intensity of 80 ╬╝mol m−2 s−1 provided by wide spectrum fluorescent tubes.