Ultimately the primary aim for the first four steps Step

In this study, the sugarcane bagasse was comprised of 48.67% cellulose, the major component, 27.13% hemicellulose and 16.35% lignin. LWH, a common used method for lignocellulose pretreatment was selected for our experiment (Van Walsum et al., 1996). Compared to dilute NVP-BGT226 pretreatment, LWH offers several potential advantages. LHW does not require acid use or transitioning to special, non-corrosive reactor materials. LHW also benefits from lower production of hydrolysate neutralization residues. Treatment of cellulose with LHW resulted in destruction of cell-wall of solid interface (Kim et al., 2009b, Li et al., 2010 and PĂ©rez et al., 2008). Therefore in Step 2 of the SCLPP, the sugarcane bagasse was pretreated using LHW to improve decomposition productivity during the SCLPP. Although the bagasse has achieved a certain degree of “decomposition” by Step 2, it still has not been fully degraded. Therefore, a third pretreatment step is necessary. For Step 3, we chose the MP method because the solid surface structure of cellulose has been damaged during Step 2. Previous studies showed that MP can lead to higher yields of reducing sugar, shorter reaction time, and lower energy consumption for pretreating starch-free wheat fibers, switch grass, and rice hulls ( Chen et al., 2012b and Janker-Obermeier et al., 2012). These benefits make MP a suitable technique for further decomposition of cellulose. The destruction of lignin structures can also be accelerated via creating nonthermal effects by the electromagnetic field in MP by Step 3. These effects may contribute to facilitating microbial growth and obtaining higher decomposition rates during subsequent pretreatment. Therefore, MD was chosen as our fourth pretreatment step of the SCLPP to further process the residual sugarcane solids.