The study observed that the oxidation slope (βa) was lower than the reductive slope (βc) at all the time intervals indicating effective oxidation of the substrate, which results in the liberation of higher number of redox equivalents ( Naresh Kumar et al., 2014). Lower redox slopes observed during the operation elucidated the efficient electron delivery with the requirement of less energy which in turn inferred the minimization of activation losses. This is an indication of the enhanced capabilities of the biocatalyst in carrying out the redox reactions in PSI-6206 microenvironment. βa was 0.057 V/dec at 0th h and showed slight decrement to 0.048 V/dec at 6th h depicting higher oxidation at 6th h. It showed a gradual increment and reached maximum at 48 h (0.171 V/dec) depicting the decrement in the oxidation reactions which is attributed to the depletion of substrate with time till the end of cycle operation and also the intermediates formed might have posed toxic environment to the microorganisms. βc showed gradual decrement from 1.097 (0th h) to 0.643 V/dec (48 h) with respect to time depicting the reduction behaviour in the reactor towards reduction of dye molecules by cleaving the azo bonds. The redox shuttles produced during the operation would have aided in the effective utilization of electrons from the place of liberation towards dye degradation.