Fermentation of the enzymatic hydrolysate containing reducing monomeric

In order to promote yeast growth, enzymatic hydrolysate was supplemented with yeast extract (10 g/L), KH2PO4 (2 g/L), (NH4)2SO4 (1 g/L) and MgSO4·7H2O (1 g/L) ( Karuppaiya et al., 2010) and medium pH was adjusted to 5.0. The mixture was inoculated with 10% (v/v) of S. cerevisiae MTCC 170 and incubated at 30 °C and 150 rpm in an incubator shaker (Scigenics Biotech, Model: Orbitek).