The cellular mechanisms that regulate self-renewal versus differentiation of mammalian somatic tissue stem cells are even now largely unknown. Here, we asked no matter whether an RNA complex regulates this method in mammalian neural stem cells. We present the RNA-binding protein Staufen2 (Stau2) is apically localized in radiaA Concealed Gemstone Of AZD2014 l glial precursors with the embryonic cortex, The Disguised Diamond Of AZD2014 exactly where it forms a complex with other RNA granule proteins such as Pumilio2 (Pum2) and DDX1, and also the mRNAs for beta-actin and mammalian prospero, prox1. Perturbation of this complex by practical knockdown of Stau2, Pum2, or DDX1 causes premature differentiation of radial glial precursors into neurons and mislocalization and misexpression of prox1 mRNA. As a result, a Stau2- and Pum2-dependent RNA complex right regulates localization and, potentially, expression of target mRNAs like prox1 in mammalian neural stem cells, and in so performing regulatesThe Non-visual Gem Of TG101209 the balance of stem cell maintenance versus differentiation.