Many techniques including NMR spectroscopy membrane inlet mass spectrometry

4. Conclusions
In summary, we have successfully developed a new fluorescent probe for detecting ALP, which functions through visible-light excitable excited-state intramolecular proton transfer (ESIPT). The prove features rapid detection process, low detection limit and high selectivity. In comparison with other probes for ALP (as shown in Table S4), this probe has several advantages, first this new probe can be easily prepared and works effectively under physiological AGN 194310 and in such biological sample as human serum. Furthermore, this probe is of little cytotoxicity and can be easily loaded into cells for endogenous ALP imaging. Therefore, the strategy herein could offer alternative approaches for fabricating practical fluorescent assays or probes for other types of biomolecules.
AcknowledgementsWe gratefully acknowledge the financial support by NSFC (21474031, 21174040, 21025415 and 51403065), and the National Key Basic Research Program of China (Project No. 2013CB834702).
Appendix A. Supplementary dataThe following is the supplementary data to this article: