Silver colloids (E and J) of different volumes (1.25–20 μL) are made up to 40 μL with DMSO (dimethyl sulfoxide) and DPPH (0.1 mM) solution is added. The reaction mixture is incubated in dark condition for 20 min at room temperature. After incubation, the decrease in the Busulfan of the mixture is monitored at 517 nm. Ascorbic acid is taken as the reference (R). The radical scavenging potential of the antioxidants is indicated by the decrease in absorbance of DPPH mixed reaction mixture on addition of the antioxidant.