Study The sixth and seventh

Fig. 6. Sex differences in housing and dietary control of motivation-related gene expression. Hamsters were subjected to social housing (SH) or social separation (SS) for 8 weeks. Accumbal mRNA levels of target Romidepsin were assessed by RT-PCR and normalized to those of beta-actin (Actb). Effects of SS and HF are described, relative to SH and LF, respectively. (A–C) Female treatments (n = 5). (A) Creb1 mRNA levels were reduced by SS during LF feeding, and elevated by HF feeding, particularly during SS. (B) SS did not alter ΔFosB mRNA levels during LF feeding, but HF feeding elevated them, particularly during SS. (C) SS did not alter Tlr4 mRNA levels during LF feeding, but HF feeding elevated them, particularly during SS. (D–F) Male treatments (n = 5). (D) SS did not alter Creb1 mRNA levels, but HF feeding reduced them. (E) SS did not alter ΔFosB mRNA levels during LF feeding, competitive exclusion and HF feeding reduced them, particularly during HF feeding. (F) SS did not alter Tlr4 mRNA levels, but HF feeding elevated them. Means ± SEM were compared by two-way ANOVA: SH vs. SS, *p < 0.050, **p < 0.010; LF vs. HF, †p < 0.050 and ††p < 0.010.Figure optionsDownload full-size imageDownload as PowerPoint slide