Total licks mean and sem by GPR

Although the DoKO mice self-infused less 20% IL than WT mice in the empty spout test, they self-infused more 20% IL than the WT mice in the training period with the food-baited sipper spout. It is possible that licking in the training sessions was limited by the satiating action of the IG 20% IL infusions and Apicidin of the GPR40/120 receptors reduced fat-induced satiation. Consistent with this view are findings implicating GPR40 and GPR120 receptors in fat-stimulated release of the satiety hormones CCK and GLP-1 [9], [16], [17], [25] and [52]. To evaluate a possible satiation deficit in GPR40/120 DoKO mice, we used a test designed to create a high level of intake of an attractive noncaloric solution, so that satiating effects can be observed as reductions in intake. We have used integumentary system method to evaluate the satiating effects of other infusions [29], [37], [38] and [39]. Naive DoKO mice and WT mice were trained to lick a 0.1% sucralose + 0.1% saccharin solution paired with matched-volume IG water infusions. The mice were then tested with the same solution paired with IG infusion of 20% IL. Both groups reduced their licking when switched from IG water to IG IL, and the group difference was not significant (Supplementary Fig. 2). The DoKO mice suppressed licking by 27% from the IG water to IG IL tests, which did not differ significantly from the 34% suppression of the WT mice. Thus, this test did not reveal a deficit in GPR40/120 DoKO mice and further evidence would be needed to document a satiation deficit in these animals.