AcknowledgmentsThe authors thank Carlo Bagnato, Ganesh Gowrishankar and Atsushi Takagi for fruitful discussions, and Wayne Dailey and Camila Shirota for editing the text. This work was supported by EU grants FP7-ICT-231724 HUMOUR, FP7-ICT-601003 BALANCE and H2020-ICT-23-2014 COGIMON.
NMDA Purmorphamine antagonist; Amnesia; Plus-maze; Cognition; ELISA
The present paper was designed to investigate whether anxiety-related behaviors and BDNF levels in the hippocampus are related to the learning/memory deficit induced by MK-801 in rats evaluated in the mEPM learning test. The duration of the effect was also analyzed.
2. Material and methods
Fifty two adult male Wistar rats weighing 260–320 g, bred in the IIBCE animal facilities (Montevideo) were employed in the androecium study. The animals were housed in groups of 6 in plastic cages (50 × 37.5 × 21 cm) with food and water available ad libitum and kept under controlled conditions (temperature 22 ± 2 °C, 12-h light–dark cycle, lights on at 7:00 A.M.). All procedures were carried out in accordance to the IIBCE Bioethics Committee's requirements and under the current ethical regulations of the national law on animal experimentation N°18.611. Adequate measures were taken to minimize animals' discomfort or stress, and all efforts were made in order to use the minimal number of animals necessary to produce reliable scientific data.