Thus, both lipid and polymers may in principle be used as building blocks for creating biomimetic membranes. This could suggest a rather low sensitivity to the nature of the host membrane. However, membrane–protein interactions may play an important role, since the mechanical properties (i.e. membrane stiffness and curvature propensity) can affect the function of transmembrane spanning Nanaomycin A , ,  and . The implications of membrane–protein interactions for AQPs are evident in a series of papers describing AQP-0 and AQP-4 reconstitution into lipid bilayers. For example, Tong et al. investigated the sorting of bovine AQP-0 into lipid microdomains in lens membranes . Lens membranes contain high concentrations of cholesterol (Chol) and sphingomyelin (SM), as well as phospholipids, such as phosphatidylcholine (PC) with unsaturated hydrocarbon chains resulting in formation of detergent resistant SM/Chol enriched microdomains in these membranes. They found that for both crude membrane fractions and proteoliposomes composed of lens proteins in phosphatidylcholine/sphingomyelin/cholesterol lipid bilayers, AQP-0 was found in both detergent resistant membrane (DRMs) and detergent soluble membranes (DSMs). Analysis of purified reconstituted AQP-0 showed that the microdomain location of AQP-0 depended on lipid-to-protein ratio (LPR). AQP-0 was located almost exclusively in DSMs at an LPR of 1200:1, whereas ~ 50% of the protein was located in DRMs at an LPR of 100:1.