The XRD patterns of the as prepared white powder after

To clarify the advantages of the peroxide-based route, the co-precipitation method without any hydrogen peroxide addition was also examined for comparison. Using this method, TCP was observed after calcination at 800 °C, which is different from the results for the peroxide-based route. Some reports have observed that the reaction model of bone with H2O2 is complex, and H2O2 acts as a deproteinating agent, leading to the reduction of the activation AY 9944 [11]. Fig. 2 presents FE-SEM images of HA prepared using the peroxide-based route after calcination at 500 °C (2a) and 800 °C (2b). Well-dispersed nanoparticles of HA can be observed in both images, approximately 10–20 nm HA was observed at 500 °C, and 30–50 nm HA was still observed at 800 °C. The particle size of HA prepared using the peroxide-based route after calcination at 800 °C (2b) was much smaller than luteinizing hormone (LH) of TCP prepared using the co-precipitation method without H2O2 (2c). The 4-times-higher BET surface area achieved using the peroxide-based route (23.54 m2/g) compared with co-precipitation (5.77 m2/g) confirms the advantage of the peroxide-based route.