Fig xA A ECL vs time curves a neither
To assess the sensitivity and the quantitative range of the proposed cytosensor, the analytical performance of the prepared cytosensor was explored by quantifying the K562 cancer GPLG0634 with different concentrations including a blank one as a reference under the optimized condition. The ECL intensity corresponding to the blank sample probably was ascribed to the physical adsorption. Fig. 5 displayed the ECL intensity responses of the BSA/aptamer/Pt-CNTs/GCE corresponding to K562 cells at different concentrations. Calibration plots displayed a good linear relationship between the ECL intensity and the logarithm of the K562 cancer cells concentration in the range from 500 to 5.0 × 106, and the equation of linear regression was ΔECL = 2222.19–5389.74 lgccell (cells mL−1), with a correlation coefficient of 0.9916. The error bar represents the SD of six independent experiments. The limit of detection (LOD) value for cancer cells was determined at 500 cells mL−1, which was partially lower than most of the other cytosensor. Compared with previous studies ( Table 1), a wider linear range and lower detection limit were both achieved for cancer cells.