So far, there is little understanding of the intrinsic effects of MWW composition on process performance, anammox Regorafenib and the overall structure of the microbial community, as observed for example in the case of source-separated urine treatment (Bürgmann et al. 2011). Only few research efforts have focused on real MWW, namely pre-settled diluted raw MWW (De Clippeleir et al. 2013) or nitrite-amended secondary clarifier effluent (Ma et al. 2013) and aerobically pre-treated MWW (Lotti et al.