Fluorescent sensors are powerful tools for visualizing and quantifying molecules and ions in living cells. A number of smaller molecule and genetically encodedLigase sensors happen to be designed for learning intracellular Zn2+ homeostasis and signaling, but no direct comparisons exist, generating it demanding for researchers to recognize http://www.selleckchem.com/products/cpi-613.html the suitable sensor for any provided application. Right here we directly review the extensively made use of little molecule probe FluoZin-3 plus a genetically encoded sensor, ZapCY2. We show that, in contrast to FluoZin-3, ZapCY2 exhibits a well-defined cytosolic localization, presents estimates of Zn2+ concentration with minor variability, doesn't perturb cytosolic Zn2+ ranges, and exhibits fast Zn2+ response dynamics. ZapCY2 was employed to measure Zn2+ concentrations in five various cell types, revealing higher cytosolic Zn2+ amounts in prostate cancer cells compared to normal prostate cells (though the total zinc is decreased in prostate cancer cells), selleck chemical suggesting distinct regulatory mechanisms.