Detection and quantification of pathogen-derived antigenic structures clearly is really a vital process for that initial diagnosis and follow-up of various infectious ailments. Complicated parasitic ailments this kind of as leishmaniasis need remarkably delicate and specific tests before remedy with probably toxic medicines. To investigate the diagnostic possible of cell surfaceInterleukin-4 receptor glycans located on Leishmania parasites, we recognized diagnostically related glycan epitopes and applied synthetic glycan microarrays to display sera from infected humans and dogs. Around the basis from the screening outcomes, we selected a tetrasaccharide to make anti-glycan antibodies. The corresponding tetrasaccharide-carrier protein conjugate was immunogenic in mice, and sera obtained from immunized mice specifically detected the Leishmania parasite. These benefits show how synthetic glycan arrays, in blend with immunological methods, assist to identify promising carbohydratemore information antigens for pathogen detection.