Detection and quantification of pathogen-derived antigenic structures selleck chemicals llc is a important method to the preliminary diagnosis and follow-up of many infectious illnesses. Complicated parasitic conditions this kind of as leishmaniasis call for highly sensitive and precise exams just before treatment with potentially toxic medication. To investigate the diagnostic likely of cell surfaceInterleukin-4 receptor glycans discovered on Leishmania parasites, we recognized diagnostically appropriate glycan epitopes and made use of synthetic glycan microarrays to display sera from infected people and canines. About the basis on the screening results, we selected a tetrasaccharide to produce anti-glycan antibodies. The corresponding tetrasaccharide-carrier protein conjugate was immunogenic in mice, and sera obtained from immunized mice exclusively detected the Leishmania parasite. These outcomes demonstrate how synthetic glycan arrays, in mixture with immunological approaches, support to determine promising carbohydrateselleck antigens for pathogen detection.