Anaerobically digested sewage sludge (10 kg) was obtained from the municipal wastewater treatment facility of the city of Larissa, Greece. Anaerobically digested sewage sludge is produced after SB 203580 digestion at mesophilic temperatures (35 °C) in anaerobic digesters of continuous flow, allowing complete mixing, and operated at high load rates. The sludge which was digested was collected from the primary settling and mixed with small amounts of sludge from the secondary settling. The sludge produced had a water content of 70% and its properties are shown in Table 1. Upon its production marine biome was stored at aerobic conditions in the municipal wastewater treatment plant prior to its collection. Anaerobically digested sewage sludge was partially dried (to 50% of its water holding capacity) and it was sieved to pass through a 3 mm mesh. LASS was collected from the secondary settlers of the municipal wastewater treatment facility of the city of Larissa, Greece. The liquid aerobic sewage sludge collected was used immediately after its collection to avoid prolonged storage which might suppress the elevated metabolic activities of the microbial biomass.