Absorption factor method AF For the determination of AT the

Different concentrations of divisor were used and the divisor concentration 24 μg/ml of AM was found to be the best regarding sensitivity, repeatability, signal to noise ratio and average recovery percent when used for the prediction of AT concentrations, in bulk powder as well as in laboratory prepared mixtures.
The second critical step is the choice of the wavelengths at which measurements were recorded. Any two wavelengths can be chosen provided that they exhibit different amplitudes in the ratio spectrum and good linearity is present at each wavelength individually.