This study demonstrates that the

This study demonstrates that the radical crosslinking copolymerization of N-vinylformamide (NVF) and divinylbenzene (DVB) in an inverse suspension, in silicone oil as the continuous phase, was effective in small polymerization vessels. The obtained copolymers of NVF and DVB had high mole percent polymerization degrees. P(NVF-co-DVB) possessed spherically sized grains of the polymer matrix, which were stable during the alkaline hydrolysis. In this reaction, the amide groups of P(NVF-co-DVB) were converted to the amine groups yielding P(VAm-co-DVB). Then, the copolymers were chemically modified with GA. This modification allowed receiving the stable P(VAm-co-DVB)/GA copolymers that after immobilization of enzyme generated systems for the Go 6976 hydrolysis. The P(VAm-co-DVB)/GA/enzyme biocatalytic systems possessing carrier–spacer–enzyme covalent imine bonds (between bifunctional glutaraldehyde and the primary amino groups of carrier and primary amines of enzyme, including the ε-amino group of lysine), called Schiff bases, were stable; thereby, confirming the physical adsorption of the enzyme on the carrier surface. Application of the Lowry’s method demonstrated that the enzyme was immobilized on the polymer carriers through bonding between primary amino groups and formaldehyde groups. All investigated copolymers were efficient carriers for cellulolytic enzymes (Novozym 476). The P(VAM-co-DVB)GA/2000/350 carrier (the enzyme covalently bonded) exhibited the highest activity among all the studied biocatalytic systems.