At injection volumes higher than 180 μL, a slight increase in the analytical signal with peak broadening was observed. On the other hand, at high injection volumes, the analysis time increased. Thus 180 μL of the sample volume was selected for a better sensitivity and shorter analysis time.
Optimization of reagent concentrations by Simplex method
Fig. 10. Responses for different trials in Simplex optimization.Figure optionsDownload full-size imageDownload as PowerPoint slide