Salmonella sp pathogen determination was performed by using the ISO

Salmonella sp. (pathogen) determination was performed by using the ISO 6579:2002/Amendment 1:2007 protocol. Briefly, after sample dilution (1:10) in BPW, the sample was incubated at 37 °C for 18 h. Then 100 μl of solution was plated on MSRV agar and incubated at 41.5 °C for 48 h. Salmonella sp. grows forming AZ5104 halo, which was then streaked onto XLD and BGA agars and incubated at 37 °C for 24 h.