Taken together these results showed no evidence of repairable or

Taken together, these results showed no evidence of repairable or unrepairable DNA damage or potential activation of detoxification processes (ahr2, cyp1a, EROD) despite parental exposure to PAHs that Ro3280 are potential inducers of these activities. These results are in agreement with those directly found in the parental generation of our offspring larvae ( Larcher et al., 2014). Indeed, these authors have recorded a high incidence of tumors related to a refractory detoxification bioactivation or DNA damage (DNA strand breaks and micronuclei) via dietary exposure, whatever the aromatic fraction considered. In parallel, a kinetic of cyp1a expression related to the larval feeding condition was performed and had demonstrated that following a 24-hour starvation period, an early activation of cyp1a occurred after a new feeding event with contaminated food, but rapidly returned to background level. This might explain the absence of genotoxic effects in parental generation and consequently in the first generation of offspring in case of genetically-based response transmission.