The goals of this study were to assess the water quality at potentially threatened sites across Missouri and to ascertain whether the type of point source contamination to surface water could be determined based on Isoprenaline activities and chemical concentrations present in the water sampled from each location. Six sites were selected for investigation, including two nearby permitted atmospheric release sites of BPA, and four downstream of current or historical WWTP effluent discharge sites (Fig. 1, Table 1). At each site, grab water samples were collected to ascertain chemicals present at a given point in time, and passive samplers were deployed to measure chemicals present in the water at the specific location over approximately 35 days. We hypothesized that 1) concentrations of BPA and EE2 would be greater near permitted airborne release sites and WWTP effluent inputs, respectively, and 2) that BPA and EE2 would be responsible for the majority of estrogenic and BPA for the majority of anti-androgenic receptor activities observed in water samples collected near the respective site types. Concentrations of BPA and EE2 were compared to observed receptor activities of individual chemical standards to assess the contribution to total receptor-based activities. Further, quantitation of a comprehensive set of wastewater compounds was performed to help characterize each site. Altogether, receptor activities and individual chemical concentrations were analyzed to determine point source pollution potential and contamination signatures.