Parallel hematoxylin and eosin staining con firmed the information on mitotic cells morphologically and pericentrin unique indirect immunofluorescence confirmed the presence of Aurora A associated supernu neverless merary centrosomes. To specify the past movement cytometric analyses, which only offered data to the total quantity of G2 M phase cells, the mitotic index was evaluated in indirect immunofluorescence examination of Aurora A and nuclear staining. For each cell line no less than one hundred cells had been counted in three independent experiments. This unveiled the highest mitotic index in OE21, followed by OE33, OE19, Kyse 410 and EPC hTERT cells. Similarly, the occurrence of multipolar mitoses was assessed by quantifying indirect immunofluorescence examination of Aurora A and nuclear stainings.
For this, in every single cell line not less than 80 mitoses have been counted in three independent experiments. Aurora A beneficial multipolar mitoses were most frequent in OE33 followed by OE21 and Kyse 410 cells. OE19 cells also as EPC hTERT cells, if any, only had single Aur ora A optimistic multipolar mitoses. Presence of supernu merary centrosomes in these multipolar mitoses was confirmed by pericentrin staining. These information propose that similarly higher Aurora A expression alone is insufficient to induce prominent Temozolomide multipolar mitoses in aneuploid esophageal cancer cells. Distinct p53 mutations contribute to multipolar mitoses in esophageal cancer cells In view on the part of p53 in post mitotic cell cycle manage, centrosome duplication and Aurora A interac tion at the same time as its frequent mutation in eso phageal carcinogenesis, we up coming determined p53 mutation status, p53 protein expression and intracellular localization during the control EPC hTERT cell line and in the 4 esophageal cancer cell lines.
The control EPC hTERT cells exhibited a wild form p53 sequence and showed weak p53 protein expression in immunoblot and indirect immunofluores cence evaluation. This wild form p53 protein was situated while in the cytoplasm of EPC hTERT cells. In contrast, all ESCC and BAC cell lines displayed p53 mutations, OE21 cells exhibited p53 muta tions in exon four, which introduce a prevent codon with the N terminus of the p53 core domain. The p53 protein of OE21 cells lacks pretty much the whole DNA binding domain, the tetrameriza tion domain along with the severe C terminus. This protein, if at all remaining expressed, is more than likely non functional considering the fact that just about all domains are missing, such as the Aurora A interaction web sites Serine 215 and 315. Without a doubt, immuno blot analysis didn't detect this largely truncated p53 protein and immunofluorescence showed only www.selleckchem.com/rac.html weak and rather diffusely localized p53 staining in OE21 cells. Kyse 410 cells displayed a stage mutation in exon 10 on the tetramerization domain.