Twin-screw extrusion cooking food trials were performed to investigate the differential effects of conventional DDGS (DDGS) and HP-DDG inclusion, seeing as fishmeal replacers, on physical properties (moisture content, water activity, thermal properties, expansion ratio, unit density, mass density, water absorption, solubility and pellet ruggedness indices, and color) of Rainbow trout (Oncorhynchus mykiss) feed. Four element blends were developed with 20 and 40% C- DDGS, and 20 and 40% HP-DDG, and also other required dietary materials. Each diet was extruded using two replications; a fishmeal based upon diet acted as the control diet. The best and the cheapest extrudate moisture content and drinking water activity were observed for the diets including 20% DDGS and 40% HP-DDG, respectively. When compared to control diet plan, increasing HP-DDG content from 20 to 40% had no effect on the majority density of the merchandise, while increasing DDGS content from 20-40% resulted in a significant rise in bulk density of the extrudates by 14.2 and 6%, respectively. As well, extrudates with the lowest lighting, greenness, and yellowness ideals were obtained from the diet containing 20% DDGS, whereas the most color strength values were observed for the diets made compounding extruder up of 40% DDGS and 20% HP-DDG. Raising DDGS and HP-DDG from 0-40% caused a remarkable increase in water absorption index, by 72 and 30%, respectively. Moreover, the normal water solubility index heightened, but in a considerably lesser purchase of magnitude. High pellet resilience index of more than 99% was attained with inclusion of either DDGS or HP-DDG. non-e of the diets impacted the thermal real estate of the extrudates. Inclusion of 20 and 40% DDGS led to the extrudates with the biggest and the tiniest unit density ideals of almost 887 and 750 kg/m3, respectively. As was expected, growth ratio decreased with raising unit density. Increasing inclusion degrees of HP-DDG didn't show any effect on unit density or growth ratio of the merchandise. Future research should focus on investigating the consequences of both extrusion digesting and graded degrees of several types of DDGS on Rainbow trout feeds.