Kinetics of biofilm development in different basal media with 3μM Fe(II) ...
Kinetics of biofilm development in different basal media with 3 μM Fe(II) evaluated by two methods: (a) CV assay and b. image capturing and 3D-pattern development (t0 = 0 h, t1 = 7 h, t2 = 31 h and t3 = 55 h).
According to the results in Fig. 6, following time evolution (t1–t3), the P. veronii 2E biofilm in presence of Fe(II) developed in thickness once the surface was totally covered. A more structured biofilm was obtained under these experimental conditions, as reported by other Pseudomonas. As found by Glick et al.  Fe-limiting conditions would be the responsible of rhamnolipid synthesis and twitching motility stimulation leading to a flat biofilm for P. aeruginosa, in Rucaparib with a structured biofilm obtained when Fe was present.
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