The first planting of Jatropha curcas saplings began in January 2009.Average monthly temperature on the web-site was 30��C with an yearly rainfall of 2200mm . The soil on the spot is often a Typic Paleudult with a sandy clay to sandy clay loam texture UNC1999 .2.2. Experimental SetupThe study started in August 2009 until eventually July 2010. Two examine plots had been established at the site. The plot that was planted with Jatropha curcas was designated as plot P although the wasteland was designated as plot S. The size of both plots is roughly 1 hectare finding subsequent to every single other with the two plots getting an roughly 7% slope.At plot P, 5 3 �� 3m quadrats had been randomly placed. Every single quadrat in plot P comprised of 4 Jatropha curcas trees. Soil sampling and soil flux measurements had been carried out within the five quadrats at plot P.
Stem diameter of each tree in every quadrat (n = twenty) was measured monthly at plot P.At plot S, five three �� 3m quadrats had been also randomly positioned. Within these quadrats, nevertheless, only soil CO2 flux measurements had been produced. One more five 3 �� 3m quadrats had been randomly positioned at plot S for the removal of native vegetation on the wasteland all through the first stage Camptothecin from the study.2.3. Biomass Determination2.3.one. Biomass at Plot P Destructive sampling of Jatropha curcas trees of different stem diameter and ages was produced to find out the allometry relationship between increments of stem diameter with increments of its biomass dry excess weight in accordance with the techniques of Basuki et al. . The destructive sampling was carried out in a Jatropha curcas plot at Universiti Putra Malaysia, Serdang, Malaysia. It had been assumed that Jatropha curcas trees at Universiti Putra Malaysia Camptothecin would be representative on the trees at Port Dickson as soil at the two websites are of the identical soil series, and both sites had been also planted with all the exact same nearby ��Sengkarang�� accession.Fifteen trees were randomly chosen and had been sawn off as shut as is possible for the ground.