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Thus, knowledge of the chemical forms of As is crucial for accurate data on the toxicological significance and geochemical cycling of As (Tchounwou et al., 2003). The inorganic As (iAs) in drinking water is generally found to be > 95% of the total As (tAs) level, which can be absorbed easily in the gastrointestinal tract (Kazi et al., 2011). Approximately 80–100% of inhaled and ingested As is absorbed through the gastrointestinal tract and lungs. However, up to 50–70% of the absorbed As is gradually eliminated by methylation in the kidneys and passed through urine. When more amounts of As are ingested than excreted, it tends to accumulate in the hair, nails, and other PF00299804 (Nielsen, 2001). For the speciation of iAs, several studies have been conducted to develop separation and pre-concentration methods (Baig et al., 2009, Baig et al., 2010a, Zhang et al., 2011 and Brahman et al., 2013). To determine the total iAs, the enrichment of the solid phase (sorbent) from the liquid phase (sample solution) is used (Zhang et al., 2005 and Zhang et al., 2004). The cloud point extraction (CPE) method has been commonly used to extract iAs species (AsIII and AsV) (Bezerra et al., 2005 and Pereira and Arruda, 2003). These sample pre-concentration methodologies are simple, cost-effective, and environmentally friendly, in addition to offering a high pre-concentration factor.