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It was also reported that eDNA originates from your intracellular DNA underneath disorders during which lysis isn't observed [15]. In case of Staphylococcus new epidermidis [16] and Enterococcus faecalis [17], eDNA final results from the autolysin-mediated killing of the smaller subpopulation of cells, to provide DNA like a part of biofilm matrix. A recent study displays that eDNA is usually launched by way of autolysis and will be a major contributor to vancomycin-enhanced biofilm formation in Staphylococcus aureus [18].Biofilm formation by A. baumannii [19, 20] has facilitated its survival on dry surface and enhanced antibiotic resistance, resulting in A. baumannii being a persistent infectious agent in intensive care units (ICUs). Our current investigation [21] demonstrates that biofilm formation by A.

baumannii strains on clinical units, this kind of as urinary catheters, could explain their potential IDO to persist in clinical environments and their purpose in device-related infections. However, the available knowledge within the unique molecular determinants inside the development of biofilms in a. baumannii is scarce. We hypothesize that eDNA could possibly be a single this kind of vital determinant. many Although studies on eDNA and its position in pure transformation in Acinetobacter happen to be reported earlier [22�C25], to date eDNA remains an uncharacterized determinant during the pathogenic bacterium A. baumannii. Because biofilm formation is actually a key virulence aspect in the. baumannii, characterization of eDNA from A. baumannii with regard to biofilm development is worthwhile investigating. In this study, we've characterized eDNA from a multidrug-resistant clinical strain of the. baumannii and demonstrated its position in in vitro biofilm formation on abiotic surfaces. two. Materials and Methods2.one. Bacterial Culture and Development ConditionsA clinical strain of Acinetobacter baumannii AIIMS 7, previously isolated [26], was used in this study.