Statistical examination was performed by Student's two tailed t-test, and P value < 0.05 was considered to be statistically significant.2.13. Nucleotide Sequence Accession NumbersThe Interleukin-11 receptor accession numbers of 16SrRNA and 5��coding region of bap sequences (amplified from eDNA) was "type":"entrez-nucleotide","attrs":"text":"HM992508","term_id":"302316221","term_text":"HM992508"HM992508 and "type":"entrez-nucleotide","attrs":"text":"HM765514","term_id":"303306205","term_text":"HM765514"HM765514, respectively.3. Results3.1. Presence of eDNA in Extracellular Growth MediumTo check the presence of DNA in the extracellular growth medium of A. baumannii AIIMS 7, eDNA was purified by isopropanol precipitation at ice-cold temperature. eDNA was found to be present along the temporal scale of A.
baumannii AIIMS seven development as much as 96 hrs and showed a pattern as shown in graph (Figure 1(b)). The pattern of eDNA (Figure one(b)) showed almost comparable concentrations of eDNA inside the early development phase (as much as 36 hrs), a fall while in the mid-stationary phase (48 hour), followed by steady concentrations inside the late growth phases (60 to 96 hrs). done Correlation of development curve (Figure one(a)) and concentration of purified eDNA showed that the prevalence of eDNA was practically steady. Concentrations of eDNA have been comparable to earlier findings in Pseudomonas aeruginosa . Figure one(a) Development curve of Acinetobacter baumannii AIIMS seven. (b) Pattern of eDNA inhibitor AR-12 release in temporal scale of a. baumannii AIIMS 7 development. Graph representing concentrations of eDNA purified from cell-free supernatant of the. baumannii AIIMS 7 at respective time ...three.2. Membrane Vesicles Existing in Extracellular Growth Medium Contained DNAMembrane vesicles had been purified from cell-free supernatant (early growth phase) and characterized employing electron microscopy to find out their dimension and morphology.