Statistical evaluation was carried out by Student's two tailed t-test, and P worth < 0.05 was considered to be statistically significant.2.13. Nucleotide Sequence Accession NumbersThe Interleukin-11 receptor accession numbers of 16SrRNA and 5��coding region of bap sequences (amplified from eDNA) was "type":"entrez-nucleotide","attrs":"text":"HM992508","term_id":"302316221","term_text":"HM992508"HM992508 and "type":"entrez-nucleotide","attrs":"text":"HM765514","term_id":"303306205","term_text":"HM765514"HM765514, respectively.3. Results3.1. Presence of eDNA in Extracellular Growth MediumTo check the presence of DNA in the extracellular growth medium of A. baumannii AIIMS 7, eDNA was purified by isopropanol precipitation at ice-cold temperature. eDNA was found to be present along the temporal scale of A.
baumannii AIIMS 7 growth up to 96 hours and showed a pattern as shown in graph (Figure 1(b)). The pattern of eDNA (Figure 1(b)) showed practically similar concentrations of eDNA in the early growth phase (up to 36 hours), a fall in the mid-stationary phase (48 hour), followed by regular concentrations in the late development phases (60 to 96 hrs). therefore Correlation of growth curve (Figure 1(a)) and concentration of purified eDNA showed the prevalence of eDNA was nearly consistent. Concentrations of eDNA had been comparable to earlier findings in Pseudomonas aeruginosa . Figure one(a) Growth curve of Acinetobacter baumannii AIIMS 7. (b) Pattern of eDNA order inhibitor release in temporal scale of a. baumannii AIIMS 7 development. Graph representing concentrations of eDNA purified from cell-free supernatant of the. baumannii AIIMS seven at respective time ...three.two. Membrane Vesicles Current in Extracellular Development Medium Contained DNAMembrane vesicles were purified from cell-free supernatant (early development phase) and characterized utilizing electron microscopy to determine their size and morphology.