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Statistical examination was carried out by Student's two tailed t-test, and P worth < 0.05 was considered to be statistically significant.2.13. Nucleotide Sequence Accession NumbersThe Interleukin-11 receptor accession numbers of 16SrRNA and 5��coding region of bap sequences (amplified from eDNA) was "type":"entrez-nucleotide","attrs":"text":"HM992508","term_id":"302316221","term_text":"HM992508"HM992508 and "type":"entrez-nucleotide","attrs":"text":"HM765514","term_id":"303306205","term_text":"HM765514"HM765514, respectively.3. Results3.1. Presence of eDNA in Extracellular Growth MediumTo check the presence of DNA in the extracellular growth medium of A. baumannii AIIMS 7, eDNA was purified by isopropanol precipitation at ice-cold temperature. eDNA was found to be present along the temporal scale of A.
baumannii AIIMS 7 growth up to 96 hrs and showed a pattern as shown in graph (Figure 1(b)). The pattern of eDNA (Figure one(b)) showed almost comparable concentrations of eDNA in the early growth phase (as much as 36 hours), a fall in the mid-stationary phase (48 hour), followed by steady concentrations from the late development phases (60 to 96 hrs). http://www.selleckchem.com/TNF-alpha.html Correlation of development curve (Figure one(a)) and concentration of purified eDNA showed the prevalence of eDNA was nearly consistent. Concentrations of eDNA have been comparable to earlier findings in Pseudomonas aeruginosa . Figure one(a) Growth curve of Acinetobacter baumannii AIIMS 7. (b) Pattern of eDNA AZ20 release in temporal scale of the. baumannii AIIMS 7 growth. Graph representing concentrations of eDNA purified from cell-free supernatant of the. baumannii AIIMS seven at respective time ...three.two. Membrane Vesicles Present in Extracellular Growth Medium Contained DNAMembrane vesicles had been purified from cell-free supernatant (early development phase) and characterized applying electron microscopy to find out their size and morphology.