.. Furthermore, serum AbIDO responses had been characterized by analyzing the pattern of IgG subclasses current in sera from mice in groups I to III. As proven in Figure 2, GO6983 phosphorylation only OVA-specific serum IgG1 Ab responses had been demonstrated in the serum from mice immunized with OVA alone (group I). On the other hand, the induction of OVA-specific serum IgG1, IgG2a, and IgG3 antibody responses was demonstrated in sera from mice in groups II and III. In particular, the production of anti-OVA IgG1, IgG2a, and IgG3 antibody was drastically enhanced through the intraperitoneal administration of SucPG-modified liposomes containing OVA (group III) than by that of OVA-containing SucPG-unmodified liposomes (group II) (IgG1, P < 0.019; IgG2a, P < 0.003; IgG3, P < 0.0091).
Figure 2Profiles of OVA-specific IgG antibody subclasses in mice intraperitoneally immunized with OVA-containing SucPG-liposomes. Mice have been immunized intraperitoneally with OVA alone (group I) or polymer-(SucPG-) unmodified liposomes entrapping OVA (group II) ...three.two. Th6 and Th6 Cytokine Production by Spleen Cells from Mice Immunized Intraperitoneally with OVA-Containing SucPG-Modified Liposomes The induction of OVA-specific serum IgG1, IgG2a, and IgG3 antibody responses by intraperitonealSCH900776 cancer immunization with OVA-containing SucPG-modified liposomes suggests effective big histocompatibility complex presentation on the antigen major to the two humoral (IgG1) (Th6) and cell-mediated (IgG2a and IgG3) (Th6) responses (Figure two). To characterize antigen-specific Th6 and Th6 responses, spleen cells have been isolated from mice provided SucPG-modified liposomes that entrap OVA (group III) and restimulated with OVA in vitro.
Culture supernatants from OVA-stimulated spleen cells were then examined to the presence of Th6 and Th6 cytokines by ELISA. As shown in Figure 3, larger amounts of both Th6 (IFN-��) and Th6 (IL-4) cytokines have been detected from the culture supernatant harvest from in vitro OVA-stimulated spleen cells from mice in group III than did spleen cells from nontreated control mice. Figure 3Th6 (IFN-��) and Th6 (IL-4) cytokine secretion by spleen cells from mice after intraperitoneal administration of OVA-containing SucPG-modified liposomes. Spleen cells have been harvested on day seven right after secondary immunization and cultured with OVA for ...3.three.
Induction of IFN-��- and IL-4-Specific mRNA in Spleen Cells from Mice Immunized Intraperitoneally with OVA-Containing SucPG-Modified Liposomes The production of Th6-type (IFN-��) and Th6-type (IL-4) cytokines by spleen cells from mice getting intraperitoneal OVA-containing SucPG-modified liposomes following in vitro restimulation was confirmed (Figures three(a) and 3(b)). To verify this locating at molecular amounts, Th6 and Th6 cytokine-specific RT-PCR was performed through the use of RNA samples extracted from spleen cells of mice intraperitoneally immunized with SucPG-modified liposomes containing OVA. Success are proven in Figure 4.