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even so, it is premature to draw any sturdy con clusions. Our study has limitations and you can find mainly related with two big variables. It's a retrospective study with a limited sample size and its lacks a molecu lar experimental part. It can be an investigative and evidence of idea study since the exact role of HEV role in cancer metastasis is still beneath investigation. The limited sample dimension of 65 individuals is derived more than a period of 5 years and it is a subset of a massive group selected from the Head and Neck support database in two big tertiary institutions. This sample population dimension was deemed to get sufficient to demonstrate a significant vary ence inside their general survival in between the 2 study groups. Nonetheless, in an work to cut back confounders and errors from the interpretation of our effects, a rigid assortment cri teria was applied to obtain a additional homogenous group.
As a result of compact sample size, we feel that a number of the associations in our hypothesis didn't reach statistical sig nificance. The retrospective nature on the review is no doubt a limitation but since the nature of this examine doesn't involve therapeutics or even a comparison of aspects, the retro spective nature truly operates to our advantage. We are able to collate a considerable sample study size with confirmatory clinic pathological data inside a brief period of time and focus our time and effort during the investigative and analytical facets. Secondly, 1 principal investigative attribute of our research concentrated largely within the histopathological evaluation of your patients LN standing preserved tissue paraffin blocks in storage.
You will find limitations to using tissue paraffin sections along with the out there procedures have their limita tions. You'll find deficiencies in the consistency in the qual ity from the tissue paraffin blocks, some tissue blocks are less properly preserved than other people, specifically the older blocks might not be stored in optimum problems over time. We use an optimized strategy for our immunohistochemis check out applying anti MECA 79 as our sole antibody for HEV. Ideally we'd want to use a minimum of two different HEV markers to verify our findings in occasion of specificity and sensitivity inaccuracies inside the antibody, nevertheless, there may be no other commercially out there antibody for HEV to date. A single assumption during the study is that MECA 79s specificity and sensitivity is substantial adequate and representative of all HEV in our tissues.
In the long run, examination on the amount of mo lecular genetics will probably be the vital element in confirming the value of immunohistochemical stains inside the assessment of biological habits and prognosis. A substantial challenge for being overcome may be the marked variation in tissue staining that may be encountered, the two in numerous, sufferers, neo plasms and in different laboratories.