Though cellobiose could be utilized in other methods hydrolysis of
Pitch-based activated carbon (AC) was kindly provided by Clean Energy Laboratory, Huazhong University of Science and Technology. The nominal Boc-D-FMK adsorption capacity of AC was 75 mg/g. Woody biochar (0.5 mm in diameter, sieved) was the by-production of gas production through a fast pyrolysis of sawdust at 800 °C in cyclone furnace with a 0.35 water flow equivalence ratio. Biochar was washed in 2% glucose solution with 2% w/v at 60 °C, 200 rpm for 3 h. After solid/liquid separation, liquid phase was removed and biochar was treated with another batch of glucose solution again. This procedure was repeated till glucose content in liquid phase remained constant. Biochar was then washed by deionized water till no glucose could be detected. Characteristics of AC and treated biochar (BC) were 1514 and 10.12 m2/g in SBET, 0.61 and 0.016 cm3/g in Vt, 0.44 and 0.0032 cm3/g in Vmic and 2.43 and 7.23 nm in Dp, respectively.
2.2. Media and growth conditions for C. thermocellum