R kratochvilovae HIMPA Genbank Acc No KF was cultivated

R. kratochvilovae HIMPA1 (Genbank Acc. No. KF772881) was cultivated at 30 °C on a rotary shaker at 200 rpm for 48 h using YEPD broth as reported earlier ( Patel et al., 2014). To obtain the seed culture, AZD7762 were harvested by centrifugation, washed twice with sterilized distilled water and resuspended in 0.9% sterilized saline to attain cell density of 6.5–7.8 × 108 cells/ml. Batch cultivations of R. kratochvilovae HIMPA1 for TAG accumulation were performed in 250 ml Erlenmeyer flasks where all fermenting (glucose, fructose, and sucrose), non-fermenting (glycerol, xylulose, and arabinose) carbon sources and mixture of both of them were standardized in moles of carbon equivalents relating to 70 g/l glucose (388 mM), mixed with salt solution (g/l): KH2PO4, 1; MgSO4, 0.5; (NH4)2SO4, 1; CaCl2, 0.1; trace elements (mg/l): boric acid, 0.5; CuSO4, 0.04; KI, 0.1; FeCl3, 0.2; MnSO4, 0.4; NaMO3, 0.2; ZnSO4, 0.4; vitamin solution (mg/l): D-Biotin, 0.002; calcium pantothenate, 0.4; folic acid, 0.002; inositol, 2; niacin, 0.4; PABA, 0.2; pyridoxine HCl, 0.4; riboflavin, 0.2; thiamine, 0.4. All experiments were done in triplicate.