In recent years biomimetic olfactory cell based biosensors have

In recent years, biomimetic olfactory cell-based biosensors have gained intensive attention and have been extensively applied in many fields such as biomedicine, food and drug safety, and environmental protection [1] and [2]. Olfactory E-64-c can recognize the specific chemical signals presented by various odorants with extreme high sensitivity, which have been increasingly recognized as promising candidates for the development of chemical sensors. Various biomimetic olfactory cell-based biosensors have been developed based on the coupling of olfactory cells with different types of transducers such as light-addressable potentiometric sensors (LAPSs) [3], [4] and [5], surface plasmon resonance (SPR) devices [6], and microelectrode array (MEA) chips [7]. Currently, one of the main challenges of this technology is how to achieve controllable and high-efficient coupling of olfactory cells with transducers which can meet the specific performance requirements for biomedical applications. At present, olfactory cells are usually directly cultured on the surface of transducers and consequently, result in the random distribution of cells and uncontrollable coupling with transducers. This obvious drawback leads to various limitations to the performances and applications of biomimetic olfactory cell-based biosensors such as low stability and poor repeatability. Therefore, to circumvent these limitations, it is highly essential and favorable to develop novel approaches to control the immobilization and distribution of olfactory cells on the surface of transducers for cell-based biosensors.