the Ridiculous Microtubule Associated Conspriracy
Osteoblasts Microtubule Associated expressing the homophilic adhesion molecule N-cadherin form a hematopoietic stem cell (HSC) niche. As a result, we examined how N-cadherin expression in HSCs relates to their perform. We identified that bone marrow (BM) cells hugely expressing N-cadherin (N-cadherin hi) aren't stem cells, being largely devoid of a Lineage(-)Scal(+)cKit(+) population and not able to reconstitute hematopoietic lineages in irradiated recipient mice. Instead, long-term HSCs form distinct populations expressing N-cadherin at intermediate (N-cadherin(int)) or minimal (N-cadherin(lo)) ranges. The minority N-cadherin(lo) population sellectchem can robustly reconstitute the hematopoietic technique, express genes that may prime them to mobilize, and predominate between HSCs mobilized from BM to spleen. The larger N-cadherin(int) population performs poorly in reconstitution assays when freshly isolated but improves in response to overnight in vitro culture. Their expression profile and lower cell-cycle entry charge recommend N-cadherinint cells are getting held in reserve. Consequently, differential N-cadherin expression reflects functional distinctions involving two HSC subpopulations.