It is reported that the tuber
The peak at 430 nm was found to be increasing with the reaction time and the variance of maximum absorbance with time is depicted in the inset of Fig. 3. The inset figure shows contractile vacuole the absorbance increases almost linearly up to 45 min of reaction time suggesting the formation of more number of nanoparticles in mixture . Beyond 90 min of incubation, the formation tends to saturate leading to the completion of reaction.